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Table 1 Accession collection of B. rapa and B. oleracea accessions for determining erucic acid contents of seeds and their FAE1 polymorphisms at coding region

From: Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents

Cultivar No. Cultivar name Erucic acid content of seeds (%) Origin Positions of nucleotides and amino acid polymorphisms
B. rapa     591a 735 898 968 1020 1265
     P197=b A245= R300= T323I K340= F422=
1 Kunshan maquedan 48.58 China G C C C G T
2 Sanyue huang 49.76 China G C C C G T
3 Hanzhong aiyoucai 45.70 China G C C C G T
4 Xinxian huangyoucai 52.02 China G C C C G T
5 7801_1 51.26 China G C C C G T
6 Jumbuck 1.93 Australia A T C T A T
7 Hja 96337 0.90 Finland A T A T G C
B. oleracea     489c 542 1079 1422 1458  
     Q163= T181K F360S S474= Y486=  
1 ChunFeng 40.75 China A A T A C  
2 Zhonggan11 45.90 China A A T G C  
3 Xiaguang Oleracea 43.27 China G C C G T  
4 Zhengchun Oleracea 47.63 China A A T A C  
5 Jingfeng2 48.20 China A C T G Y  
6 Xinfeng 41.40 China A A Y G Y  
7 Zaofeng 51.52 China A A Y A Y  
8 Improved         
  Niuxin Oleracea 42.07 China A A T A C  
9 Hanchun3 41.39 China G A T G C  
  1. a591 indicates the position 63 bp from the base 'A' in the start codon of B. rapa FAE1. P197=b indicates amino acid diversity due to this nucleotide polymorphism. c489 indicates the position 63 bp from the base 'A' in the start codon of B. oleracea FAE1. Three haplotypes of FAE1 can be inferred among these seven B. rapa accessions. Accessions Nos. 1-5 are B. rapa haplotype 1; accession No. 6 is B. rapa haplotype 2 and accession No. 7 is B. rapa haplotype 3. Seven haplotypes of FAE1 can be inferred among these nine B. oleracea accessions. Accessions Nos. 1, 4 and 8 are B. oleracea haplotype 1; Accessions No. 2, 3, 5, 6, 7 and 9 are B. oleracea haplotypes 2 to 7, respectively.