Regions of the B. napus FAE1 genomic sequences amplified by the primer pairs. Lines represent 5' and 3' genomic sequences of FAE1 (~1 kb 5' and 3' for both Bn.FAE1-A8 and Bn.FAE1-C3) and the rectangles represent the coding region (1-521 bp). FAE1-A8 (line with triangles at the end) indicates the specific primer for Bn.FAE1-A8, and FAE1-C3 (line with diamonds at the end) indicates the specific primer for Bn.FAE1-C3. ECOT-1 and ECOT-2 (lines with circles at the end) both amplify the Bn.FAE1-A8 and Bn.FAE1-C3 coding regions (with a short part of the 5' or 3' regions). Sequences alignment for the region of specific primers (reverse primers of FAE1-A8 and FAE1-C3) were showed in corresponding areas.