|
P450F1
|
TGAAAATTAACCAGCCACCAT
|
Gene and cDNA cloning
|
|
P450R1
|
TTATATCATTTGTGAAAGC GACAAG
|
Gene and cDNA cloning
|
|
P450F2
|
TGCATGGACTGATGCAGAC
|
Expression detection in gel
|
|
P450R2
|
CTCCTCATTCATGTCCAACTC
|
Expression detection in gel
|
|
P450F3
|
TGGAGTTGCTCAGCAGCCATA
|
Internal primer for DNA sequencing
|
|
P450R3
|
TATATGGAA GCCATCGACTGTG
|
Internal primer for DNA sequencing
|
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5'UTRF1
|
TGCAGCTTCATATCTTGGGTTTTCTC
|
5'UTR cloning (1000 bp)
|
|
5'UTRR3
|
GGTGGCTGGTT AATTTTCAGTATTCAG
|
5'UTR cloning (1000 bp)
|
|
3'UTRF1
|
ATATAATCTCTGTATCATTGCCAACTGAG
|
3'UTR cloning (600 bp)
|
|
3'UTRR1
|
ATGCTAAACATCAAATCGAATACTCTCA
|
3'UTR cloning (600 bp)
|
|
SYBR-P450F1
|
TGCATGGACTGATGCAGAC
|
Real-time qPCR for gene expression
|
|
SYBR-P450R1
|
CTCCTCATTCATGTCCAACTC
|
Real-time qPCR for gene expression
|
|
SYBR-P450F2
|
CCAACATCAAAGCTATATCTTTG
|
Real-time qPCR for pre-mRNA splicing
|
|
SYBR-P450R2
|
TGGAGGCTGCCATCAT ATC
|
Real-time qPCR for pre-mRNA splicing
|
|
P450-5'RACE GSP2
|
CGATACTTTTTGGATAGAGCTTGTAGAGC
|
First 5'RACE
|
|
P450-5'RACE GSP3
|
CGACAAGCTCCCTAACATATGCATATT
|
Second 5'RACE
|
|
P450-3'RACE GSP1
|
CGAGCAACTTGTTCATTGCTTTGAT
|
First 3'RACE
|
|
P450-3'RACE GSP2
|
CGAAGA TAACATGTTGGCAAGTGAGTT
|
Second 3'RACE
|
|
SYBR-P450B 5'UTRF1
|
ACTGACTTCTAGTTTAAATTTTTCTT
|
Real-time qPCR for 5'RACE
|
|
SYBR-P450B 5'UTRR1
|
TGGCTGGTTAATTTTCAGTATT
|
Real-time qPCR for 5'RACE
|
|
SYBR-P450B 3'UTRF1
|
ATATAATCTCTGTATC ATTGCCAA
|
Real-time qPCR for 3'RACE
|
|
SYBR-P450B 3'UTRR1
|
ACTACCAGATGAAAATCATTAAAT
|
Real-time qPCR for 3'RACE
|
|
P450-5'RACE GSP3
|
CGACAAGCTCCCTAACATATGCATATT
|
5' primer extension
|
|
P450-3'RACE GSP2
|
CGAAGA TAACATGTTGGCAAGTGAGTT
|
3' primer extension
|
|
-300UF
|
AGTCATATATGACTTAGCAAGAGAACTCCAC
|
PCR for pre-mRNA splicing at -300 TSS
|
|
-140UF
|
AACCGCACCTTATC CTCTTCACAA
|
PCR for pre-mRNA splicing at -140 TSS
|
|
-60UF
|
TGAAACCACCCAAGAACTTCAAAAC
|
PCR for pre-mRNA splicing at -60 TSS
|
|
++70UR
|
CATAAAGCATAAAGCGTTATTATTCATATTT
|
PCR for pre-mRNA splicing at ++70 poly(A) site
|
|
++200UR
|
CTAAGTTCCATATTCT TCCTTCTTAAGTTCA
|
PCR for pre-mRNA splicing at ++200 poly(A) site
|
|
++260UR
|
ACTCAGAACTAAGTTTTATTTCACTTGATCAA
|
PCR for pre-mRNA splicing at ++260 poly(A) site
|
