Figure 4
ATP and ADP measurements, mitochondrial membrane potential analysis and respiration assay in YtA line and transgnic plants. A ATP and ADP levels from the root of YtA and YtB. B ATP/ADP ratios of the plant materials. C Purified mitochondria were isolated from the root of YtA and YtB, and the mitochondrial potentials were assayed. D Respiration rates in roots from YtA and YtB. Experiments were replicated three times. E ATP and ADP levels from 3-day-old etiolated seedlings of transgenic and non-transgenic rice. F ATP/ADP ratios of the transgenic plants and non-transgenic plants. G Mitochondrial membrane potential of these plant materials. H Respiration rates in roots from wild-type and transgenic plants at one week. Three individual samples of each line were measured. The data are given as mean ± SE (n = 3). *, a to d indicate the difference between YtA and YtB is significant (p < 0.05). *, e to h indicate the difference between transgenic and wild type plants is significant (p < 0.05). U6-1T1,U6-2T1 and U3-2T1, three independent T1 progeny transgenic plants.