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Figure 3 | BMC Plant Biology

Figure 3

From: Identification of novel genes potentially involved in somatic embryogenesis in chicory (Cichorium intybus L.)

Figure 3

Effects of the β-1,3-D Yariv treatment during SE induction. A and B: Effects of β-1,3-D Yariv treatment on the occurrence (white columns) of reactivating cells (RC), fully reactivated cells (FRC) and somatic embryos (SE) in K59 explants at d4 and d11. All RC are not able to go through the second phase of cell reactivation to reach FRC status, however, the majority of FRC will form somatic embryos at d11. When β-1,3-D Yariv is applied, the numbers of RC and FRC are significantly decreased, and no somatic embryos are produced at d11. C and D: The Yariv reagent is clearly detected as an orange coloration within leaf tissue and it can be observed surrounding reactivating cells (arrows). Three micrometer semi-thin sections observed under Nomarski phase contrast. Bar = 20 μm. Hatched columns = control conditions (absence of β-1,3-D Yariv). The Student-Newman-Keuls test was applied on data collected from 10 independent slides. For one event, different letters indicate significant differences. Bars represent standard errors.

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