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Figure 2 | BMC Plant Biology

Figure 2

From: Identification of novel genes potentially involved in somatic embryogenesis in chicory (Cichorium intybus L.)

Figure 2

Characterisation of phenotypes observed in chicory leaf explants from the responsive K59 genotype and the non-responsive C15 genotype, cultured in absence (control condition Y-), or in presence (Y+) of β-Glc Y. A: Leaf explants of K59 at d0. B: Leaf explants of K59 at d11,Y-. Somatic embryos (e) can be observed at explant borders. Roots (r) can be observed during embryo development. C: Leaf explants of K59 at d11,Y+. Yariv reagent penetrates within the leaf blade. No morphogenetic pattern can be detected. D: leaf explants of K59 cultured for 4 days in presence of β-Glc Y and transferred to new medium deprived of β-Glc Y during 7 days (d11, 4Y+). Cut explant borders curl upwards and somatic embryos (e) can be observed. E: Leaf explants of C15 at d0. F: Leaf explants of C15 at d11,Y-. No morphogenetic pattern can be detected. G: Leaf explants of C15 at d11,Y+. Yariv reagent penetrates within the leaf blade. No morphogenetic pattern can be observed. H: leaf explants of C15 cultured for 4 days in presence of β-Glc Y and transferred to a new medium deprived of β-Glc Y during 7 days (d11, 4Y+). No somatic embryos can be detected. Bar = 2 mm.

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