Figure 4
Changes in metabolic pathways as affected by Fe status. Pathways related to metabolites and proteins were integrated according to the KEGG database. Statistical t-student tests were performed to both protein and metabolite data to show statistically relevant changes between samples. Red squares indicate metabolites showing significant, marked (more than 4-fold) up-accumulation compared to the controls, and yellow ones mean significant, moderate (less than 4-fold) up-accumulation. Blue squares mean significant, marked (more than 4-fold) down-accumulation, and green ones mean significant moderate (less than 4-fold) down-accumulation. The same colour code was used with proteins, represented by circles. Red circles are proteins detected only in Fe-deficient gels, and yellow ones mean significantly up-accumulated proteins using a 2-fold threshold. Decreased (at least 50%) and missing proteins in the Fe-deficient treatment were represented in green and blue respectively. Each number or letter corresponds to those shown in the corresponding protein and metabolite tables (Tables 1 and 2 respectively). Panels correspond to root tip extracts from plants Fe-deficient (A), 24h after Fe resupply (B), and 72h after Fe resupply (YZ in panel C and WZ in panel D).