Figure 1

2-D IEF-SDS PAGE proteome maps of root tips from Fe-sufficient and Fe-deficient Beta vulgaris plants. Proteins were separated in the first dimension in linear (pH 5-8) IPG gel strips and in the second dimension in 12% acrylamide vertical gels. Scans of real typical gels of root tips from Fe-sufficient and Fe-deficient plants are shown in A and B, respectively. Virtual composite images, showing all polypeptides present in root tips from Fe-deficient and Fe-sufficient plants are shown in panels C and D. Statistical significance was assessed with a t-test (p < 0.10), and a 2-fold change in spot intensity between treatments was used as a threshold. Only spots that fit both criteria are marked in panel C; spots whose intensities decrease or disappear completely with Fe deficiency were labelled with green and blue marks, respectively, and those increasing with Fe deficiency or only present in Fe-deficient gels were labelled with yellow and red marks, respectively. In panel D, polypeptides that had significant homologies with proteins in the databases (using MALDI-MS and MASCOT) are marked by squares and labelled with lower-case letters. Homologies are described in detail in Table 1.