Figure 3

Northern analysis of Minicyclamen transgenic lines. A) Schematic diagrams of the T-DNA regions of binary vectors, pLN96, pPN48, pPN50 or pPN51. These binary vectors harboured in their T-DNAs the cyclamen antisense F3'5'H gene under a CaMV35S promoter and either nptII or hpt selectable marker genes under a NOS promoter. B) Northern RNA blot analysis of hpt selectable marker expression in control and transgenic lines of cv 'Purple' (left) and RT-PCR analysis of nptII selectable marker expression of cv 'Wine-Red' (right). The expected size of the hpt signal was 1.4kb and the expected size of the nptII signal was 600bp. C) Northern RNA blot analysis of sense and antisense CpF3'5'H transcript in transgenic and control lines of cv 'Purple' (left) and cv 'Wine-Red' (right).