Figure 2

Identification and microdissection of individual tissue types from tangential cryosections. (Top) Schematic diagram and image of a stem cross-section showing an overview of tissue types. Letters A, B, C and D in the schematic indicate tangential cryosection planes corresponding to the figure panels (A), (B), (C) and (D) below. (A-D) Tissue images of tangential cryosections at different stem depths. For each horizontal figure panel (A) - (D), the left image shows tissue cryosection prior to laser cutting; the centre image shows the same tissue cryosection after laser cutting; and the right image shows the laser microdissected tissues in collection tube. Tissue types are indicated by the color bar on the left side of panels (A) - (D) where colors correspond to the colors in the schematic of the cross section (Top): Brown is cortex, yellow is cortical resin duct (CRD), orange is phloem, green is cambium zone (CZ), yellow is xylem, and grey is pith. (A) Tangential cryosection containing cortex and CRD tissues. (B) Tangential cryosection containing cortex and phloem tissues. (C) Tangential cryosection containing cortex, phloem, and cambium zone tissues. (D) Tangential cryosection containing phloem, cambium zone, and xylem tissues. CRD: Cortical resin duct; CZ: Cambium zone. Length of scale bar is 200 μm for the cross-section and 400 μm for tangential panels (A) - (D).