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Figure 1 | BMC Plant Biology

Figure 1

From: Laser microdissection of conifer stem tissues: Isolation and analysis of high quality RNA, terpene synthase enzyme activity and terpenoid metabolites from resin ducts and cambial zone tissue of white spruce (Picea glauca)

Figure 1

Schematic overview of sample preparation for laser microdissection of spruce stems. (A) 2-year old white spruce harvested above the root. (B) Stems were divided into 5 - 10 mm long pieces that were flash frozen in liquid nitrogen. (C) Stem pieces were split in half longitudinally prior to cryosectioning. (D) Half stem segment mounted for tangential cryosectioning. (E) Tangential cryosections on a PET-membrane frame slide. (F) Laser microdissection: Regions of interest are selected, cut with a laser and selected cells fall by gravity into a collection tube.

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BMC Plant Biology

ISSN: 1471-2229

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