Intricate environment-modulated genetic networks control isoflavone accumulation in soybean seeds

BMC Plant Biology

Table 2 Additive QTLs for genistein and daidzein accumulation in soybean seeds of Essex × PI 437654.

Name^a	Interval	IC^b	A ± SE^c	F-value^d	P-Value	h²(a)^e
Genistein
qGEN5*	SATT236-SAT_271	27.4-34.4	82.1 ± 5.2	35.2	0.00000	5.5
qGEN2*	SAT_279-EACAMCTT123	0.0-6.0	54 ± 4.3	34.7	0.00000	2.0
qGEN15*	SAT_112-SATT691	8.0-11.0	58.9 ± 4.6	24.8	0.00000	2.3
qGEN13*	SATT490-SAT_197	3.4-11.0	25.3 ± 4.4	13.1	0.00000	1.3
qGEN12	SCTT009-SATT541	0.0-6.0	41.9 ± 4.3	11.9	0.00000	1.2
qGEN20	GMLPSI2-SCT_189	72.5-74.5	-32.5 ± 4.1	8.1	0.00000	0.4
qGEN19*	SAT_113-SAT_286	141.4-149.8	-47.5 ± 4.5	13.3	0.00000	1.9
qGEN7*	SATT175-EAGGMCTT095	76.1-83.1	42.9 ± 4.9	14.7	0.00000	2.3
qGEN4	SAT_337-SAT_140	6.2-17.6	-47.5 ± 7.2	19.8(17.3)	0.00000	3.3
qGEN2_2	SAT_139-SAT_289	107.6-126.2	-44.8 ± 9.0	27.2(17.3)	0.00001	5.3
Daidzein
qDAI5*	SATT174-SATT236	21.5-28.4	50.3 ± 3.2	32.8	0.00000	8.3
qDAI8*	SATT187-EAGGMCTT205	23.0-25.1	-27.9 ± 2.9	17.5	0.00000	2.2
qDAI4	SATT396-SAT_337	0.0-6.0	-27.5 ± 3.2	15.7	0.00000	1.2
qDAI1	SAT_106-AW781285	34.7-42.6	20.9 ± 3.1	14.2	0.00000	1.2
qDAI2*	SAT_279-EACAMCTT123	0.0-8.0	27.5 ± 3.2	20.4	0.00000	1.4
qDAI12*	SCTT009-SATT541	0.0-3.0	63.6 ± 3.1	25.2	0.00000	4.3
qDAI16	SAT_339-SATT280	0.0-15.0	-58.8 ± 3.9	15.5	0.00000	2.3
qDAI7*	SATT175-EAGGMCTT095	75.1-83.1	55.7 ± 3.5	13.6	0.00000	2.4
qDAI7_2	SAT_147-SAT_330	145.7-151.7	-38.5 ± 3.1	9.2	0.00000	1.0

^aName given to a particular QTL, GEN and DAI for genistein and daidzein content, respectively, followed by the chromosome number and a number when more than one in the same chromosome. Asterisks highlight QTL reported in our previous study [14]. ^bInterval of confidence in centiMorgans with respect to the first marker in the LG. ^cMain additive effect in μg/g plus/minus standard error. Mean effect of substituting both Essex alleles by PI437654 alleles. Thus, positive values indicate that the PI437654 allele increase the phenotypic value. ^dF-values of significance of each QTL. Threshold F-values were 7.4 and 7.5 for genistein and daidzein, respectively, for the 4-environmentcombined analysis. Between brackets is the threshold F-value for QTLs identified at a particular environment. P-values represent the significance of each effect. ^eh²(a) is the heritability of the additive effect or percentage of variation (%) that is explained by the additive component of the QTL.

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