Nematode demographic assay for SCN resistance in roots transformed with amiRNA silencing the Rhg1 -locus LRR-kinase gene. Ratio of total infecting nematode population that gained appearance of J3 and J4/adult female stages two weeks after inoculation onto A. rhizogenes-transformed roots. Williams 82 (susceptible) and Fayette (resistant) soybean tissue (left two bars) were transformed using A. rhizogenes carrying the pSM103 negative control plasmid; Fayette + amiRNA received the designated amiRNAs that target the Rhg1-locus LRR-kinase gene (amiRNA LRR-K I and amiRNA LRR-K II). Graph shows mean ± std error of mean from three independent nematode infection assays that used roots from three independent transformation experiments (overall n > 18 for each treatment). P values above each bar are color-coded for contrasts with the bar of the designated color (null hypothesis: the observed ratio between the two treatments was similar; tested by ANOVA using SAS Proc Mixed).