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Figure 4 | BMC Plant Biology

Figure 4

From: A nematode demographics assay in transgenic roots reveals no significant impacts of the Rhg1locus LRR-Kinase on soybean cyst nematode resistance

Figure 4

amiRNA-mediated silencing of expression of the Rhg1 -locus LRR-kinase gene. A, Semi-quantitative RT-PCR to monitor expression level of the Rhg1-locus LRR-kinase gene Glyma18g02680.1/TC204550 (LRR-K) in Fayette transgenic roots generated using pSM103-derived plasmids expressing amiRNA GmLRR-K I or amiRNA GmLRR-K II, or GFP7-positive Fayette transgenic roots generated using the negative control vector pSM103 (vector control), or GFP-negative Fayette hairy roots. Actin expression was monitored in parallel from the same samples as a control. Results from three independent roots are shown for each construct; gels were stained with ethidium bromide. Similar results were observed in a second experiment using independently derived roots. B, Relative band intensity of semi-quantitative RT-PCR products. Data from the experiment shown in part A and from a similar but independent transformation experiment are shown. Band intensities were normalized to the mean value for the three Fayette control roots from the same experiment. Mean ± std error of mean are shown; statistical analysis was performed on the normalized data. Roots expressing amiRNA GmLRR-K I exhibited a significantly reduced mRNA abundance for the Rhg1-locus LRR-kinase gene, as indicated with the * (ANOVA; Tukey comparison of means; p = 0.0023).

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