Figure 1

Nematode demographics assay for SCN resistance in roots transformed with a full-length Rhg1 -locus LRR-Kinase gene. A, B, C, Representative examples of SCN developmental stages on transgenic soybean hairy roots 15 days after infection; cleared root sections were stained with acid fuchsin, which stains nematodes as well as root vascular bundles. A, Early parasitic second-stage juveniles (J2). B, Late parasitic J2s and early J3s. C, J4 or adult female. D, Ratio of total infecting nematode population that developed to J4 or adult female stage two weeks after inoculation onto A. rhizogenens-transformed roots of Ina, E98076 (both transformed with empty vector), or E98076 + LRR-K Ina (transformed with the Glyma18g02680.1 Rhg1-locus LRR-Kinase promoter/gene/terminator). Mean ± std error of mean; data combined from two independent experiments; bars with the same letter above are not significantly different (ANOVA, Tukey pairwise comparison, p < 0.001). E, Similar to D, except that "rhg1^- Rhg4⁺are putative rhg1^-/ rhg1^-; Rhg4⁺/ Rhg4⁺progeny lines from Ina × E98076 that were genotyped as carrying the E98076 (susceptible) allele of a marker linked to Rhg1 and the Ina (resistant) allele of a marker linked to Rhg4. All the lines were transformed with empty vector except "rhg1^- Rhg4⁺+ LRR-K Ina" was transformed with Rhg1-locus LRR-kinase gene (including native promoter and terminator). Mean ± std error of mean are from two independent experiments; bars with the same letter above are not significantly different (ANOVA, Tukey pairwise comparison, p < 0.05).