Figure 6

Immunodetection of AcH4 in sections of buds in phase A and B of development in longitudinal axis using a confocal microscope. (a) Differential Interference Contrast (DIC) of bud in phase A (10×). (b) DAPI (blue signals) superposition and AcH4 (green signals) of bud in phase A (10×). (c) DAPI labelling of nuclei of bud in phase A (10×). (d) AcH4 labelling of bud in phase A (10×). (e) DIC of bud in phase A (40×). (f) DAPI (blue signals) superposition and AcH4 (green signals) of bud in phase A (40×). (g) DAPI labelling of nuclei of bud in phase A (40×). (h) AcH4 labelling of bud in phase A (40×). (i) DIC of bud in phase B (10×). (j) DAPI (blue signals) superposition and AcH4 (green signals) of bud in phase B (10×). (k) DAPI labelling of nuclei of bud in phase B (10×). (l) AcH4 labelling of bud in phase B (10×). (m) DIC of bud in phase B (40×). (n) DAPI (blue signals) superposition and AcH4 (green signals) of bud in phase B (40×). (o) DAPI labelling of nuclei of bud in phase B (40×). (p) 5-mdC labelling of bud in phase B (40×).