Fig. 3

(A) Preparation of anise leaf explant. (B) A callus of an anise leaf was developed within 21 days. (C) A suspension medium, MS containing 2 mg/L BA and 2 mg/L 2,4-D, was used to start the suspension calli. (D) The anise suspension culture has been subcultured after three weeks. (E) After transferring the growing callus from subcultures to MS, a solid nutrient medium containing 2 mg/L BA + 2 mg/L 2,4-D, (F) was developed