Fig. 4

Ecotopic transient expression of CgEC124 decreased the disease resistance in maize. A Transcript level of CgEC124 in FoMV:GFP-VOX and FoMV:CgEC124-VOX plants. Bar indicates the standard deviation of three replicates. B Chitin-induced ROS burst in FoMV:GFP-VOX and FoMV:CgEC124-VOX plants. Bar indicates the standard deviation of three replicates. C The expression levels of ZmPR1, ZmPR4, ZmPR5, and ZmWRKY33, in FoMV:GFP-VOX and FoMV:CgEC124-VOX plants. Statistical significance was determined with Student’s t-test, ^****p < 0.0001. D Phenotype of FoMV:GFP-VOX and FoMV:CgEC124-VOX plants inoculated with WT strain CgTz-3. Scare bars represented 1 cm. E Statistical analysis of the lesion area and relative fungal biomass. Statistical significance was determined with Student’s t-test, ^*p < 0.05, ^**p < 0.01