Fig. 3

CgEC124 is required for the pathogenesis of C.graminicola. A Colony morphology of CgTz-3, ΔCgEC124#5 and ΔCgEC124#6, and complementation strain ΔCgEC124#6-C grown on CM and PDA medium. B Statistical analysis of the colony diameters and conidiation. Bar indicates the standard deviation of three replicates. Significance analysis was analyzed by Student’s t-test and ns means no significance. C Pathogenicity verification of ΔCgEC124 mutants and complementation strain in maize detached leaves. Photos were taken after 5 days post inoculation (dpi). D Statistical analysis of the lesion area and relative fungal biomass. Statistical significance was determined with Student’s t-test, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001