Fig. 5

Protein and protein interaction between OsNAR2.1 and OsPLDα1. A: Testing the interaction of OsNAR2.1 and OsPLDα1 using the DUAL pairwise interaction kit with ADE2 and HIS3 as reporter genes. Yeast strain NMY51 carried each pair of bait and prey plasmid, co-expression of OsNAR2.1 and OsNRT2.3a as a positive gene control for protein interactions (Yan et al., 2011; Liu et al., 2014), pBT3-C and pPR3-N are the control vectors with no cloned cDNA. Cells grown on selective SD/-Ade/-His/-Leu/-Trp or control SD/-Leu/-Trp medium. B: Bimolecular fluorescent complementary assays to detect the interaction between OsPLDα1 and OsNAR2.1. The ORFs of OsPLDα1 and OsNAR2.1 were constructed on the PSAT1-cEYFP-N1 vector and PSAT1-nEYFP-C1 vector, respectively. Fluorescence could only be detected when OsPLDα1-cEYFP was combined with nEYFP-OsNAR2.1. Bars, 10 μm