Fig. 7From: Characterization of SIPs-type aquaporins and their roles in response to environmental cues in rice (Oryza sativa L.)Expression analysis of OsSIP1, OsSIP2 with temperature response marker genes OsCFB3 (cold) and OsHSP14.7 (heat) under extreme temperature stress treatment. Two-weeks-old rice seedlings (O. sativa L. cv. Nipponbare) were treated separately at 4 °C or 42 °C. Whole plants were collected for qRT-PCR analysis after 3, 6, and 9 h treatments. Values represent the mean ± SD of three biological replicates and three technical replicates, and the Y-axis represents the relative expression level normalized to OsACTIN. Asterisks indicate significant differences (**P < 0.01, and ***P < 0.001) based on Student t-test compared to CK (0 h time point without treatment)Back to article page