Fig. 2From: Overexpression of the WRKY transcription factor gene NtWRKY65 enhances salt tolerance in tobacco (Nicotiana tabacum)Subcellular localization and transactivation activity assay of NtWRKY65 protein. (a) Subcellular localization of NtWRKY65 protein in Nicotiana benthamiana epidermal cells. (b) Transactivation activity assay of the NtWRKY65 protein. The full-length coding sequence of NtWRKY65 was inserted into the pGBKT7 vector, and transformed yeast clones were selected on SD/-Trp and SD/-Trp-His-Ade media. pGBKT7-53 was used as the positive control. Empty pGBKT7 was used as the negative controlBack to article page