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Fig. 7 | BMC Plant Biology

Fig. 7

From: A novel O-methyltransferase Cp4MP-OMT catalyses the final step in the biosynthesis of the volatile 1,4-dimethoxybenzene in pumpkin (Cucurbita pepo) flowers

Fig. 7

Expression of His-tagged Cp4MP-OMT in Nicotiana benthamiana. A SDS-PAGE and B western blot analyses of crude extract of Nicotiana benthamiana leaf tissue with (+) and without (-) recombinantly expressed His-tagged Cp4MP-OMT. Tobacco leaves were transfected with an expression vector either including the sequence for Cp4MP-OMT (+) or without it (-). C SDS-PAGE and D western blot analyses of His-tagged Cp4MP-OMT purification from crude extract. Lanes are indicated as follows: supernatant (sup), column flow through (ft), wash step (w), and elution (elu). The marker is a molecular mass marker (in kDa). Cp4MP-OMT bands were visualized using an antibody against His(6)-tag (arrows)

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BMC Plant Biology

ISSN: 1471-2229

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