Fig. 3
From: Fine mapping of TFL, a major gene regulating fruit length in snake gourd (Trichosanthes anguina L)
Genetic mapping of the gene regulating fruit length in snake gourds. (A) Distribution of the Euclidean distance (ED) association values on chromosomes (chr). The abscissa indicates the chr name, the coloured dots represent the ED value of each single nucleotide polymorphism (SNP) locus, the black line represents the fitted ED value, and the red dotted line represents the significance association threshold. The higher the ED value, the better the association effect of the SNP locus. (B) Fine-mapping of the fruit length gene using InDels. Using 2,548 F2 individuals resulting from the S1 × S2 cross, the location of the gene regulating fruit length was narrowed down to a 2.7-M region between the markers, SG60 and SG62, on chr 4. (C) Genotyping of recombinant plants from 6,000 F2 individuals resulting from the S1 × S2 cross. The fruit length of the two parents and their F1 offspring and the genotype of the recombinant single parent are shown on the right. The location of the gene that regulates the length of the fruit was narrowed down to an 18-kb region. (D)Tan0010544 (TFL) contains the c.202 C > G mutation in the coding sequence and p.Gln68Glu substitution in the protein sequence. (E) Alignment of Tan0010544 (TFL) homologs from different species. The E68Q mutation in S1 is highlighted in red