Table 1 Primer sequences used for RT-PCR
Gene | Forward primer (5’–3’) | Reverse primer (5’–3’) | bp | Tm (°C) |
|---|---|---|---|---|
StPAL | TCGAGGACGAATTGAAGGCT | CAGGGTTGCCACTTTCAAGC | 75 | 60 |
StCHS | TGATTGAAGCATTCCAACCAATAG | GTTCAACTTGGTCAAGAATTGCC | 96 | 60 |
StF3’H | CGTCAACGTTTGGGCCATTT | TTCACCTCCGGGCAAGAATC | 88 | 60 |
StDFR | ACCCAAAGCACTGCAGAC | CTGGTGCATTCTCCTTGCC | 88 | 60 |
StANS | TGAGTCCCTCCTAAAGGCCA | TCATGTTTCCGCGTTCTCCA | 86 | 60 |
StUFGT | TGAATGCGTTTGCGTTGGTT | ACATGCTCATATTTCCCCACGA | 82 | 60 |
StGAPDH | ATGAAGGACTGGAGAGGTGG | GAAAATGCTTGACCTGCTGT | 75 | 60 |