Comparative analysis of chloroplast genomes of Pulsatilla species reveals evolutionary and taxonomic status of newly discovered endangered species Pulsatilla saxatilis

Table 5 Relative synonymous codon usage (RSCU) for protein-coding genes in Pulsatilla saxatilis

Codon	AA	ObsFreq	RSCU	Codon	AA	ObsFreq	RSCU	Codon	AA	ObsFreq	RSCU
GCA	Ala	419	1.13	AAG	Lys	391	0.53	AGC	Ser	131	0.37
GCC	Ala	251	0.68	CUA	Leu	387	0.82	AGU	Ser	392	1.12
GCG	Ala	189	0.51	CUC	Leu	206	0.44	UCA	Ser	447	1.27
GCU	Ala	618	1.67	CUG	Leu	188	0.4	UCC	Ser	348	0.99
UGC	Cys	95	0.59	CUU	Leu	602	1.27	UCG	Ser	212	0.6
UGU	Cys	229	1.41	UUA	Leu	853	1.81	UCU	Ser	579	1.65
GAC	Asp	233	0.4	UUG	Leu	598	1.27	ACA	Thr	441	1.24
GAU	Asp	925	1.6	AUG	Met	658	1	ACC	Thr	253	0.71
GAA	Glu	1068	1.47	AAC	Asn	309	0.46	ACG	Thr	171	0.48
GAG	Glu	385	0.53	AAU	Asn	1045	1.54	ACU	Thr	558	1.57
UUC	Phe	554	0.72	CCA	Pro	336	1.15	GUA	Val	562	1.51
UUU	Phe	984	1.28	CCC	Pro	230	0.78	GUC	Val	190	0.51
GGA	Gly	742	1.58	CCG	Pro	159	0.54	GUG	Val	206	0.55
GGC	Gly	199	0.42	CCU	Pro	448	1.53	GUU	Val	535	1.43
GGG	Gly	327	0.7	CAA	Gln	736	1.51	UGG	Trp	481	1
GGU	Gly	608	1.3	CAG	Gln	239	0.49	UAC	Tyr	202	0.4
CAC	His	157	0.46	AGA	Arg	507	1.79	UAU	Tyr	817	1.6
CAU	His	526	1.54	AGG	Arg	193	0.68	UAA	*	44	1.47
AUA	Ile	762	0.95	CGA	Arg	394	1.39	UAG	*	25	0.83
AUC	Ile	486	0.61	CGC	Arg	112	0.39	UGA	*	21	0.7
AUU	Ile	1159	1.44	CGG	Arg	130	0.46
AAA	Lys	1089	1.47	CGU	Arg	368	1.3

ISSN: 1471-2229