Table 3 Genetic fidelity analysis of invitro regenerated plants of apple root stock MM 104 using ISSR and SCoT marker
Primers used (ISSR and SCoT) | Primer sequence (5’ to 3’) | No. of scorable bands | No. of bands | Percentage | Band size (kb) | |||
|---|---|---|---|---|---|---|---|---|
Monomorphic | Polymorphic | Monomorphism | Polymorphism | |||||
ISSR 801 | (AT)8 T | 7 | 7 | - | 100 | - | 2.5–0.5 | |
ISSR 803 | (AT) 8 C | 6 | 6 | - | 100 | - | 1.5–0.5 | |
ISSR 808 | (AG) 8 C | 5 | 5 | - | 100 | - | 2.0–0.5 | |
ISSR 811 | (GA) 8 C | 5 | 4 | 1 | 80 | 20 | 2.0–0.5 | |
ISSR 828 | (GT)8 A | 8 | 8 | - | 100 | - | 2.5–0.2 | |
ISSR 830 | (TG) 8 G | 7 | 7 | - | 100 | - | 2.0–0.1 | |
ISSR 841 | (GA)8 YC | 5 | 5 | - | 100 | - | 2.7–0.2 | |
ISSR 845 | (CT) 8 RG | 7 | 7 | - | 100 | - | 2.5–0.5 | |
ISSR 848 | (CA)7CRG | 6 | 6 | - | 100 | - | 2.5–0.2 | |
ISSR 849 | (GT)8 YA | 8 | 8 | - | 100 | - | 2.5–0.2 | |
SCoT1 | CAACAATGGCTACCACCA | 4 | 4 | - | 100 | - | 1.5–0.2 | |
SCoT 2 | CAACAATGGCTACCACCC | 5 | 5 | - | 100 | - | 1.8–0.5 | |
SCoT 4 | CAACAATGGCTACCACCT | 4 | 4 | - | 100 | - | 1.5–0.5 | |
SCoT 5 | CAACAATGGCTACCACGA | 8 | 7 | 1 | 87.5 | 12.5 | 1.5–0.2 | |
SCoT 8 | CAACAATGGCTACCACGT | 4 | 3 | 1 | 75 | 25 | 1.5–0.2 | |
SCoT 9 | CAACAATGGCTACCAGCA | 3 | 3 | - | 100 | - | 1.5–0.5 | |
SCoT 12 | ACGACATGGCGACCAACG | 5 | 5 | - | 100 | - | 2.0–0.3 | |
SCoT 15 | ACGACATGGCGACCGCGA | 9 | 9 | - | 100 | - | 1.5–0.2 | |
SCoT 16 | ACCATGGCTACCACCGAC | 3 | 3 | - | 100 | - | 1.5–0.2 | |
SCoT 24 | CACCATGGCTACCACCAT | 4 | 4 | - | 100 | - | 1.5–0.2 | |
SCoT 26 | ACCATGGCTACCACCGTC | 10 | 10 | - | 80 | 20 | 1.5–0.2 | |
SCoT 33 | CCATGGCTACCACCGCAG | 5 | 5 | - | 100 | - | 1.5–0.2 | |