Fig. 2

Transcriptomic profiles of genes putatively involved in the FAs biosynthetic pathway. (A) Sketch of FAs biosynthetic pathway. Acetyl-CoA undergoes a series of reactions to produce C18:0-ACP, followed by desaturation to produce C18:1, C18:2, and C18:3. C18:1 can be further extended to produce VLCFA. Copyright permission of KEGG pathway maps (map00061) was obtained from Kanehisa laboratory by using the copyright permission request form in www.kegg.jp/kegg/kegg1.html. (B & C) Expression of (B) FADs and (C) KCSs involved in the FAs biosynthesis. The scales refer to log₂FPKM. (D & E) The phylogenetic trees of (D) AtruFADs and AtFADs, and (E) AtruKCSs and AtKCSs. The phylogenetic trees of AtruFADs and AtFADs, and AtruKCSs and AtKCSs were constructed using the Maximum Likelihood method based on WAG + G and LG + G + I model respectively, within MEGA software (version X) and Evolview (http://www.evolgenius.info/evolview.html). The triangle marks the protein from A. truncatum