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Table 2 Quantitative determination of phenolic acids and flavonoid compounds of L. arabicus L. callus treated with DPS (0, 2, 4, 8, 10 g/l) by using high-performance liquid chromatography (HPLC)

From: Upregulated synthesis and production of bioactive compounds in Lotus arabicus L. by in vitro feeding with dried powder of date palm seeds

No

Category

Compounds

0

2 g/l

4 g/l

8 g/l

10 g/l

RT

µg/ml

min

1

Phenolic

Rosmarinic acid

0.45 ± 0.02e

0.63 ± 0.01d

1.24 ± 0.02b

1.43 ± 0.04a

0.85 ± 0.03c

11.9

2

Caffeic acid

1.38 ± 0.8d

2.17 ± 0.3cd

5.41 ± 0.35a

4.04 ± 0.67b

2.43 ± 0.25c

6.09

3

Syringic acid

0.27 ± 0.01ab

0.18 ± 0.01cd

0.34 ± 0.03a

0.22 ± 0.08bc

0.11 ± 0.02d

6.49

4

Coumaric acid

0.07 ± 0.01b

0.23 ± 0.06a

0.11 ± 0.03b

0.19 ± 0.04a

0.21 ± 0.03a

8.90

5

Vanillin

0.23 ± 0.05d

0.49 ± 0.01c

0.75 ± 0.03b

0.97 ± 0.07a

0.74 ± 0.02b

9.76

6

Ferulic acid

0.07 ± 0.01b

ND

0.07 ± 0.01b

0.09 ± 0.02a

0.10 ± 0.01a

10.0

7

Chlorogenic acid

6.14 ± 0.34c

13.76 ± 0.5b

15.59 ± 1.8b

21.39 ± 2.3a

15.7 ± 1.5b

4.15

8

Cinnamic acid

0.12 ± 0.01c

0.14 ± 0.04bc

0.18 ± 0.05abc

0.21 ± 0.02a

0.19 ± 0.04ab

19.3

9

Flavonoids

Catechin

0.67 ± 0.1b

1.13 ± 0.8ab

3.05 ± 1.9a

3.12 ± 1.5a

1.28 ± 0.9ab

4.62

10

Rutin

0.62 ± 0.2ab

0.68 ± 0.1ab

0.70 ± 0.14ab

0.79 ± 0.08a

0.52 ± 0.08b

6.8

11

Naringenin

0.13 ± 0.05b

0.33 ± 0.06b

1.59 ± 0.06a

1.89 ± 0.9a

2.43 ± 0.7a

10.29

12

Quercetin

0.57 ± 0.05d

0.33 ± 0.04c

0.56 ± 0.01c

0.88 ± 0.03a

0.76 ± 0.08b

17.12

13

Kaempferol

1.62 ± 0.6a

ND

0.36 ± 0.03c

0.97 ± 0.05b

1.15 ± 0.2ab

20.3

14

Daidzein

0.13 ± 0.03b

0.11 ± 0.04b

0.24 ± 0.01a

0.09 ± 0.01b

0.21 ± 0.08a

16.07

Total content

20.18 ± 1.1d

19.07 ± 0.6c

30.19 ± 2.4b

36.28 ± 0.3a

26.68 ± 2.14b

 
  1. The data are means ± SE. Different small letters indicate statistically significant differences between different treatments according to Duncan’s test (P < 0.05)