Fig. 7

Phylogenetic and conservation analysis of AIL5. a Phylogenetic tree of LcAIL5 homologous proteins. The unrooted phylogenetic tree was constructed using the maximum-likelihood (ML) method in IQ-TREE. Isoform structure was constructed using GSDS 2.0. Blue structure, UTR region; yellow structure, CDS; red box, variable exon. b Conserved protein domain alignment of isoforms with SE splicing event. c Base bias analysis of alternative exon and splice site of AIL5 genes. d Relative expression of LcAIL5_AS1 and LcAIL5_AS2 in different developmental stages. The error bar shows Std. deviation