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Fig. 6 | BMC Plant Biology

Fig. 6

From: Combined sensory, volatilome and transcriptome analyses identify a limonene terpene synthase as a major contributor to the characteristic aroma of a Coffea arabica L. specialty coffee

Fig. 6

DHS-GC–MS analyses of the N. benthamiana leaves volatile compounds following transient expression. N. benthamiana leaves were transformed with terpene synthase 10-like (35S:TPS10-like (Coffea arabica)) and two other mono-TPSs that have been shown to be involved in limonene production. TPS1 (35S:TPS1 (Coffea arabica)) was characterised as a limonene synthase in Coffea arabica by Del Terra et al. [13]. It was found to be expressed in drupes 25 weeks after pollination and was used here as a positive control. TPS7 (35S:TPS7 (Solanum lycopersicum)) was characterised as a beta-myrcene/limonene synthase in Solanum lycopersicum by Zhou & Pichersky [40]. Leaves of N. benthamiana were transformed with a TPS construct (35:TPS10-like, 35S:TPS1, 35S:TPS7) or with the 35S:pBIN61 empty vector for the negative control. For each construct, three independent biological replicates were used to quantify relative amounts of volatile compounds. Relative amounts are expressed as mean percentage of total peak areas; individual values are indicated with black dots; bars indicate the standard error. Means with different letters are significantly different (Tukey’s HSD test, P < 0.01)

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BMC Plant Biology

ISSN: 1471-2229

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