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Fig. 8 | BMC Plant Biology

Fig. 8

From: Genome-wide characterization of the bHLH gene family in Gynostemma pentaphyllum reveals its potential role in the regulation of gypenoside biosynthesis

Fig. 8

Interaction of GpbHLH15 and GpbHLH58 proteins with gypenoside biosynthesis gene promoters. A Y1H assays demonstrate the binding of GpbHLH15 to the GpSS1 promoter, whereas GpbHLH58 exhibits binding to the promoters of GpFPS1, GpSS1, and GpOSC1. AD indicates a negative control using the pGADT7 empty vector. The transformed yeast cells were diluted thrice, with 10–1 dilutions each time and then spotted on the selective medium. B Dual-luciferase assays were performed using GpFPS1, GpSS1, and GpOSC1 promoters driving the Firefly luciferase (LUC) gene as reporters, along with effectors (GpbHLH15, GpbHLH58). These results reveal that GpbHLH15 activates the promoter of GpFPS1, while GpbHLH58 promotes the expression of GpFPS1, GpSS1, and GpOSC1

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BMC Plant Biology

ISSN: 1471-2229

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