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Fig. 7 | BMC Plant Biology

Fig. 7

From: Mathematical kinetic modelling followed by in vitro and in vivo assays reveal the bifunctional rice GTPCHII/DHBPS enzymes and demonstrate the key roles of OsRibA proteins in the vitamin B2 pathway

Fig. 7

Riboflavin content in rice callus overexpressing AtRibA1 and OsRibA1 and OsRibA2. As negative controls, OsRibA1 and OsRibA2 were also used with a point mutation leading to an early stop codon (indicated as OsRibA1* and OsRibA2*). Twelve independent lines, positive for each transgene, were randomly pooled and stored at -80°C until riboflavin extraction. Riboflavin was detected and quantified by HPLC–MS. Data shown correspond to means ± standard deviation of the means (SD), N = 12. Data was compared to control values (WT) by One-way ANOVA (**, P < 0.01)

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BMC Plant Biology

ISSN: 1471-2229

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