Fig. 5

Functional complementation of the yeast mutants rib1∆ (GTPCHII deficient) and rib3∆ (DHBPS deficient) with OsRibA1 and OsRibA2. The mutants transformed with the empty vector (EV) were used as negative control with no exogenous supply of riboflavin, while the wild type transformed with the empty vector (EV) and no added thiamin was used as positive control for all experiments. The growth rate was determined by calculating the slope of the non-linear regression curve applied to the exponential phase of the growth curves. A Cell spotting assay and growth rate for rib1∆, deficient in GTPCHII. B Cell spotting and growth rate for rib3∆, deficient in DHBPS. Data shows that RibA2 fully recovers the GTPCHII mutant and that RibA1 fully restores DHBPS mutant. Data shown correspond to means ± standard deviation of the means (SD) (N = 5). Data was compared to control values (WT) by One-way ANOVA (**, P < 0.01; ****, P < 0.0001)