Fig. 1

Growth of Arabidopsis seedlings exposed to different concentrations of Pi for 14 days. a Photos of different modalities. Scale bar = 1 cm. b Shoot and root fresh weight (mg) and shoot-to-root ratio per seedling. c Shoot and root total free-Pi content (ng Pi/mg fresh weight; malachite green method). d Relative gene expression of selected molecular markers. All values are relative to control (0 µM Pi roots or shoots), which is normalized to 1. AtTUBULIN was used as housekeeping gene. Bars indicate means ± SD. Different letters indicate significant different means (one-way ANOVA followed by SNK test, p < 0.05, n = 2–4 biological replicates, each replicate corresponds to a pool of different seedlings, weight has been extrapolated per seedling). *More independent experiments have been performed that displayed the same tendency, data not shown