Fig. 5From: Identification of miRNAs-mediated seed and stone-hardening regulatory networks and their signal pathway of GA-induced seedless berries in grapevine (V. vinifera L.)Validation of target genes cleaved by VvmiRNAs using RLM-RACE and PPM-RACE. The gray dotted lines indicate the cleavage sites of VvmiRNAs on target genes identified by 5′ and 3′-ends of mRNA fragment using PPM-RACE and RLM-RACE, respectively. The blue and red regions denote the sequences of VvmiRNAs and complementary sequences of VvmiRNAs on their target genes. Number above the blue regions denote the cleavage counts by RLM-RACE, while the number below the red regions indicate the cleavage counts by PPM-RACE. Each assay was repeated the three timesBack to article page