Fig. 5

sqRT-PCR of Medicago truncatula ST1, ST2 and ST3 transcripts along development. a Transcript accumulation in 24 hpi seeds and in 3-, 6- and 10-d-old dark-grown seedlings (roots and aerial parts). b Transcript accumulation in 3-, 6- and 10-d-old light-grown seedlings (roots and aerial parts). c Transcript accumulation in several organs of adult plants: root from 30-d-old plants; leaves, flowers and pods in 3 developmental stages; and green seed. The different development stages of leaves were: Leaf 1, small, closed folioles without spot; Leaf 2, fully expanded leaves, folioles with spot smaller than 1 cm; and Leaf 3, fully expanded leaves, folioles with spot bigger than 1 cm. The different developmental stages of flowers were: Flower 1, green immature with sepals covering the organs; Flower 2, closed yellow flower two days before anthesis; and Flower 3, at anthesis. The different developmental stage of pods were: Pod 1, early pod with a complete spiral; Pod 2, pod with 5 complete spirals and initial spines with embryo at globular state; and Pod 3, pod with 6 complete spirals and mature spines with embryo at heart state. Green seeds are seeds collected from 24 to 26 d after pollination. c1 Detail of bars between 0 and 50 units showed in c (red square). Transcript accumulation was measured relative to ubiquitin as indicated in the Methods section. Scale units express normalized and integrated absorbance (nIA). More detailed description of plant material is included in Additional file 3. Statistical analyses were performed separately for each ST gene as stated in the Methods section. The different letters on the tops of the histograms indicate a statistically significant difference of p < 0.05