Fig. 6

Transcript levels in in vitro grown B73 maize kernels subjected to sucrose starvation and recovery (2012 season). RT-qPCR was performed on total RNA extracted from kernel tissue using oligonucleotide primers specific for various genes involved in sugar metabolism, sensing and transport. White squares indicate kernels subjected to 48 h of sucrose starvation followed by recovery for 48 h on 150 mM sucrose. Black squares indicate kernels grown on continuous sucrose. The shaded area on the graph represent the period of sucrose starvation. The white are shows the recovery period on 150 mM sucrose. Significance as reported by the Student’s T-test is at α = 0.05 for 3 biological replicates, and indicated by asterisks. Standard error is shown with vertical bars