Fig. 7From: Transcriptional analysis of defense mechanisms in upland tetraploid switchgrass to greenbugsReal-time qPCR validation of gene expression. Expression levels of select genes up/down regulated in the RNA-Seq datasets were analyzed by real-time qPCR. The input RNA used for RNA-Seq studies were used as the source material for qPCR analyses. Gene annotation is provided in the first column and separated by functional classes: metabolism, redox, defense and transcription factors (TFs). The second column lists the correlation coefficients for individual gene expression between RNA-Seq and qPCR analyses. The Log2-fold change (infested/control) for genes on 5 (cyan), 10 (yellow), and 15 DAI (magenta) respectively are shown. Gene ids, primers, and amplicon sizes are provided in Additional file 2: Table S2Back to article page