Fig. 2

Representative two-dimensional electrophoresis (2-DE) gel maps of total leaf proteome of grapefruit leaves extracted from ⁻Las/⁻Heat, ⁺Las/⁻Heat, ⁻Las/⁺Heat or ⁺Las/⁺Heat plants. Three-year-old similarly-sized healthy or Las-infected plants were either unexposed or exposed to thermal treatment of 40 °C for 6 days in a growth chamber. A total of 300 μg of protein was loaded on a pH 4–7 IpG strip and protein spots were visualized by staining with Coomassie Brilliant Blue (CBB). M _r, relative molecular weight; pI, isoelectric point