Fig. 7
Course of soluble protein in leaf and stem base with cuttings of Petunia hybrida, grown at two Nd fertilisation levels (N low, N high) to donor plants and exposed to different environment conditions – immediately planted for rooting and planted for rooting after dark – and sampled at five exposition times (0, 6, 24, 72, 168 hpin, corresponding days are shown in the x-axis: 3d, 7d). (Panels a to d): Full cycles and open squared symbols connected with full and intermittent lines represent AR formation for 168 hpin directly after excision (0 hpe) and after dark exposure of cuttings (168 hpe, 10 °C), respectively. Multifactorial ANOVA’s revealed significant interaction effects for Nd-level × dark exposure × exposition time p < 0.05 (Panels c-d), for dark exposure × exposition time x tissue type (Panels a-b). Vertical bars represent 95 % confidence intervals of mean values. Further details of experiments and statistics are presented in methods and with Additional file 1) Experiments of nitrogen preconditioning of cuttings, Additional file 2) Explanation of experimental designs for statistical analyses and Additional file 3) Supplemental data of figure 7 for Exp. 8: PR-NDCR, respectively