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Fig. 5 | BMC Plant Biology

Fig. 5

From: The functional divergence between SPA1 and SPA2 in Arabidopsis photomorphogenesis maps primarily to the respective N-terminal kinase-like domain

Fig. 5

DS_122-HA associates with cry1 and cry2 in blue light. a, b Co-immunoprecipitation of cry1 (a) and cry2 (b) by DS_122-HA. 4-day-old dark-grown seedlings (D) were transferred to 50 μmol m−2 s−1 B for 1 h (B). DS-122-HA was expressed in spa1 spa2 spa3 and under the control of the SPA2 promoter. Col-0, SPA2::SPA1-HA and SPA1::SPA2-HA lines were used as controls. SPA-HA proteins were immunoprecipitated using α-HA beads. α-HA antibody was used to detect SPA-HA proteins. α-cry1 and α-cry2 antibodies were used to detect cry1 and cry2, respectively. Asterisks likely indicate phosphorylated cry1 and cry2. To obtain similar SPA-HA protein levels from different transgenic lines in B, all seedlings were treated with proteasome inhibitor to reduce protein degradation in B. Also, SPA2-HA was expressed under the control of the stronger SPA1 promoter to counteract the strong B-induced degradation of SPA2. Five times more protein extract was used for the SPA1::SPA2-HA 64 and DS_122-HA 27 co-immunoprecipitations than for the SPA2::SPA1-HA 28 and DS_122-HA 37 co-immunoprecipitations

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BMC Plant Biology

ISSN: 1471-2229

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