Fig. 2
The diverged part of the N-terminal domain of SPA contributes to the divergence of SPA1/SPA2 function in the light. a The top row shows a schematic representation of amino acid identity between SPA1 and SPA2 domains. Below indicates the chimeric SPA1/SPA2 domain swap proteins DS_NT12-HA and DS_NT21-HA carrying swaps in the N-terminal domains of SPA1/SPA2. All chimeric constructs were expressed in a spa1 spa2 spa3 mutant background under the control of the SPA2 promoter and fused with a C-terminal HA tag. NLS indicates the site of a predicted nuclear localization sequence. b, c Phenotype of 4-day-old spa1 spa2 spa3 mutant seedlings expressing DS_NT12-HA or DS_NT21-HA swap constructs. Representative T2 seedlings are shown. Seedlings were grown in darkness or in 0.05 μmol m−2 s−1 FRc (b) and in 0.01 μmol m−2 s−1 Rc or 0.05 μmol m−2 s−1 Bc (c) for 4 days. Numbers indicate independent transgenic lines