Mapping QTLs and association of differentially expressed gene transcripts for multiple agronomic traits under different nitrogen levels in sorghum

Table 1 Descriptive statistics, h ², and mean squares of ANOVA results for the traits measured across two normal-N conditions in CK60 x China17 RIL population

Category	Source of variation	Df	Chl1	Chl2	Chl3	PH	AD	MC1	MC2	BY	GY	TW	GS
Descriptive statistics	CK60		49.8	55.6	53.6	99	71.5	68.6	24.8	7.69	2.89	20.3	0.52
	China17		46.6	52.7	48.3	150	66.3	65	19.5	14.6	6.25	31.6	0.95
	RIL Mean		47.8	53.3	47.2	161.3	67	66.5	19.4	11.2	3.39	23.6	0.47
	Std		4.09	3.72	5.85	35	4.2	3.32	6.34	3.99	1.49	3.15	0.16
	Min		38.7	38.2	32.3	70	55.1	52.8	8.16	3.09	0.4	14.4	0.05
	Max		58.4	62.2	62.5	236.5	85.9	76.1	46.8	24.2	9.04	29.9	0.88
	h² (%)		71	56	51	64	61	40	53	62	55	64	39
	SE (%)		6	9	10	7	8	12	9	0.8	9	7	12
ANOVA	Env	1	626.9	4276***	17016***	40478	11333***	347.6	4500	271.6	32.8*	946.9	0.07
	Rep(Env)	2	89.1*	9.93	57.8	10976**	55.6*	191.1**	2501***	34.6	2.57	1483***	0.02
	Blk(Env*Rep)	44	12.5	12.0*	40.4***	429*	11.3	14.8	31.7	13.1	2.43*	5.69	0.02
	Line	130	50.9***	41.9***	105.3***	4037***	49.6***	34.3**	123.9***	49.4***	6.8***	28.0***	0.08**
	Env*Line	104	15.6**	18.1***	58.2***	1634***	20.5**	21.8**	59.3***	19.6***	3.2***	10.5***	0.048***
	Residual	190	9.74	8.06	16.7	290	12.8	12.7	26.7	10.5	1.54	5.2	0.02

Df, degrees of freedom; chlorophyll contents at vegetative stage (Chl1), at anthesis (Chl2), and at maturity (Chl3); PH, plant height (cm)
AD, days to anthesis; MC1, % stover moisture content; MC2, % head moisture content; BY, biomass yield (t.ha⁻¹); GY, grain yield (t.ha⁻¹)
TW, test weight (g); GS, grain/stover ratio (%). Std, standard deviation; h² (%), narrow sense heritability; SE (%), standard error %; ***P < 0.0001; **P < 0.01; *P < 0.05

ISSN: 1471-2229