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Table 2 Mutation analysis of nontransgenic T2 triple mutant plants

From: A CRISPR/Cas9 toolkit for multiplex genome editing in plants

T1 lines

NT T2 triple mutant lines

ETC2

TRY

CPC

A17

A17-1

+A/+A

+C/+C

+T/+T

A17-2

+A/+A

+C/+T

+T/+T

A17-3

+A/+A

+G(×2)/+T(×8)

+A/+T

A17-4

+A/+A

+C(×6)/+T(×3)

+C/+T

A17-5

+A/+A

+C/+C

+T/+T

A17-6

+A/+A

+C/+T

+C/+T

A17-7

+A/+A

+C/+T

+T/+T

A17-8

+A/+A

+T(×5)/+T(×5)

+T/+T

A17-9

+A/+A

+T/+T

+C/+T

A33

A33-1

+C/+C

-C(×4)/-C(×4)

+G/+G

A33-2

+A/+C

-C/-C

+G/+G

A33-3

-TCG/-TCG

+T/+T

+A/+A

  1. Two types of mutations from direct sequencing of PCR products were obtained based on double-peaks on chromatograph. “+” indicates insertion, “–” indicates deletion. Two alleles are separated by “/”. For mutations identified by sequencing of DNA from clones harboring PCR products, the number of clones harboring the same mutation is indicated in parentheses.