| | No. 5'-tagged sequences |
---|
| | TAIL-PCR | I-PCR | Different sequencesa |
---|
ET2-Line | T-DNA copies | AD2 | AD2-1 | AD2-5 | BsrGI | BclI | |
---|
17 | 5 | 3 | 5 | 2 | 2 | 2 | 5 |
156 | 5 | 2 | 1 | 2 | 2 | 2 | 3 |
49 | 4 | 4 | 5 | 1 | NT | 4 | 6 |
179 | 4 | 2 | 3 | 3 | 3 | 5 | 4 |
85 | 3 | 2 | 1 | 1 | 2 | NT | 3 |
111 | 3 | 2 | 3 | 1 | 1 | 3 | 2 |
34 | 1 | 1 | 2 | 1 | 2 | 2 | 1 |
Average | 3.3 | 2.3 | 2.9 | 1.6 | 2.0 | 3.0 | 3.4 |
- The minimum number of T-DNA copies was determined by Southern hybridization with a DIG-labelled luc+ probe. Isolation of 5'-tagged sequences was accomplished using three different arbitrary degenerated (AD) primers in TAIL-PCR and two different restriction enzymes in I-PCR.
- aNumber of different 5'-tagged sequences obtained.
- NT, not tested.