From: Phenotypic and fine genetic characterization of the D locus controlling fruit acidity in peach
BACend marker | Primer sequence (5'-3') | Size* (bp) | Polymorphism | Annealing temp. (Ā°C) | Enzyme |
---|---|---|---|---|---|
F109-15-06 | F GTAGGATGAACTCAAAGGTG | 570 (J, F) | SNP | 52 | Tsp509I |
Ā | R GTTGGTAATGACACTGGCTA | Ā | Ā | Ā | Ā |
R109-15-06 | F GTGGACTTCATCCCATCTAC | 540 (J, F) | SNP | 54 | HincII |
Ā | R GGTCCAGAAGATGATGCAC | Ā | Ā | Ā | Ā |