Figure 6

Detection of AtNPR1 and VvNPR1.1 transgene expression in grapevine leaves. Leaves from in vitro grown V. vinifera cv Syrah were infiltrated with A. tumefaciens transformed with pBIN+ carrying AtNPR1 or VvNPR1.1. Control plants were infiltrated with water. Infiltrated leaves were challenged with P. viticola 3 days after agroinfiltration. Total RNAs were extracted 3 days after agro-infiltration (uninoculated) and 2 days after P. viticola inoculation. Full-lenght mRNA from each transgene was specifically amplified after reverse transcription with primers listed in table 1. VvACT was used as internal control.