Figure 3
Expression patterns of VvNPR1.1 and VvNPR1.2 during a compatible or an incompatible interaction between grapevine and Plasmopara viticola. Leaves of plantlets of Vitis vinifera cv Chardonnay (grey bars) and Vitis riparia cv Gloire de Montpellier (dark bars) were inoculated with Plasmopara viticola (1.5 × 105 spores mL-1). Control leaves were sprayed with water. Leaves were collected at different time points as indicated. Hpi: Hours post inoculation. Transcript levels of each gene (Stilbene synthase VvSTS (A); VvNPR1.1 (B); VvNPR1.2 (C)) were normalized to actin transcript levels. The fold induction indicates normalized expression levels in inoculated leaves compared to normalized expression levels observed in water-treated leaves at the same time point. Expression ratio at the beginning of the experiment (0) is set to 1. Mean values and standard deviations were obtained from 2 duplicate experiments.