Figure 1

Comparison of VvNPR1.1 and VvNPR1.2 with other NPR1 homologs and members of Arabidopsis thaliana NPR family. (A) Phylogenetic tree generated with the Phylo_win program using the neighbour-joining method [44]. Sequence alignment was previously realized using the ClustalW tool. Accession numbers are: AtNPR1 (At1g64280), AtNPR2 (At4g26120), AtNPR3 (At5g45110), AtNPR4 (At4g19660), AtBOP1 (At3g57130), AtBOP2 (At2g41370), Nicotiana tabacum (NtNPR1, AAM62410.1), Oryza sativa cv. japonica (OsNPR1, AAX18700.1), Lycopersicon esculentum (LeNPR1, AAT57637.1), Musa acuminata (MNPR1A, ABI93182.1; MNPR1B, ABL63913.1), Malus × domestica (MpNPR1-1, ACC77697.1) and Vitis vinifera (Genoscope accession numbers: VvNPR1.1, GSVIVP00016536001; VvNPR1.2, GSVIVP00031933001). Bootstrap values based on 500 replicates are indicated beside the branches. (B) Schematic representation comparing the structure of AtNPR1, VvNPR1.1 and VvNPR1.2, including the positions of the BTB/POZ domain, the ankyrin repeat domain (ARD) and the nuclear localization signals (NLS). (C) Multiple alignment of putative nuclear localization signals (NLS) at C-terminus of NPRs from different plant species. Basic amino acids are highlighted in grey and residues essential for AtNPR1 nuclear localization [30] are highlighted in black.